Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates
Braz. j. microbiol
; 41(2): 278-282, Apr.-June 2010. tab, ilus
Artículo
en Inglés
| LILACS
| ID: lil-545329
Biblioteca responsable:
BR32.1
ABSTRACT
The emergence of Extended-Spectrum Beta-Lactamase (ESBL)-producing microorganisms in Brazilian hospitals is a challenge that concerns scientists, clinicians and healthcare institutions due to the serious risk they pose to confined patients. The goal of this study was the detection of ESBL production by clinical strains of Escherichia coli and Klebsiella sp. isolated from pus, urine and blood of patients at Hospital Universitário Santa Maria, Rio Grande Sul, RS, Brazil and the genotyping of the isolates based on bla SHV genes. The ESBL study was carried out using the Combined Disc Method, while Polymerase Chain Reaction (PCR) was used to study the bla SHV genes. Of the 90 tested isolates, 55 (61.1 percent) were identified as ESBL-producing by the combined disk method. The bla SHV genes were found in 67.8 percent of these microorganisms. K. pneumoniae predominated in the samples, presenting the highest frequency of positive results from the combined disk and PCR.
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Contexto en salud:
Enfermedades Desatendidas
Problema de salud:
Enfermedades Desatendidas
/
Zoonosis
Base de datos:
LILACS
Asunto principal:
Pacientes
/
Pseudomonadaceae
/
Beta-Lactamasas
/
Farmacorresistencia Microbiana
/
Secuencia de Bases
/
Reacción en Cadena de la Polimerasa
/
Enterobacteriaceae
/
Infecciones por Enterobacteriaceae
/
Pruebas Enzimáticas Clínicas
Tipo de estudio:
Estudio diagnóstico
/
Estudio de prevalencia
/
Factores de riesgo
Límite:
Humanos
Idioma:
Inglés
Revista:
Braz. j. microbiol
Asunto de la revista:
Microbiologia
Año:
2010
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Federal de Santa Maria/BR
/
Universidade de Santa Cruz do Sul/BR