Detección de mutaciones asociadas a cepas multidrogo resistente de Mycobacterium tuberculosis en Chile / Detection of genes associated with drug resistance in Mycobacterium tuberculosis strains isolated in Chile
Rev. méd. Chile
; 139(4): 467-473, abr. 2011. ilus
Article
en Es
| LILACS
| ID: lil-597642
Biblioteca responsable:
BR1.1
ABSTRACT
Background:
The incidence of acquired resistance to antituberculous drugs of Mycobacterium tuberculosis in Chile is approximately 23 percent.Aim:
To analyze the mutations associated with drug resistance in drug resistant strains of Mycobacterium tuberculosis. Material andMethods:
In 28 drug resistant Mycobacterium tuberculosis strains isolated in Chile, genes leading to drug resistance were studied. DNA was amplifed by polymerase chain reaction (PCR) and sequencing was carried out using the ABI PRISM big dye terminator cycle sequencing ready reaction kit.Results:
In rifampicin-resistant strains, the mutations in rpoβ gene were in the codons S531W/L (56 percent), D516Y (16 percent) and D516V (16 percent). The predominant mutation in katG gene was in the codon S315L (73 percent) in isoniazid-resistant strains. The mutation S95T was found in the 71 percent of ciprofoxacin resistant strains. Only one ethambutol resistant strain had the M306I mutation. Three unreported mutations in katG were identifed.Conclusions:
Drug resistance associated mutations of Mycobacterium tuberculosis isolated in Chile were similar to those reported abroad.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Farmacorresistencia Bacteriana Múltiple
/
Genes Bacterianos
/
Mutación
/
Mycobacterium tuberculosis
/
Antituberculosos
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
/
Risk_factors_studies
Límite:
Humans
País/Región como asunto:
America do sul
/
Chile
Idioma:
Es
Revista:
Rev. méd. Chile
Asunto de la revista:
MEDICINA
Año:
2011
Tipo del documento:
Article
País de afiliación:
Chile