Survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction
Mem. Inst. Oswaldo Cruz
; 106(6): 763-768, Sept. 2011. tab
Artículo
en Inglés
| LILACS
| ID: lil-602063
Biblioteca responsable:
BR1.1
ABSTRACT
This work reports a survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction (PCR). Seventy pampas deer were captured in the dry season and surveyed using PCR, microscopic agglutination test (MAT) (n = 51) and by both techniques (n = 47). PCR detected infections in two pampas deer and MAT detected infections in three. Through sequencing and phylogenetic analyses, the PCR-amplified fragment detected in deer was identified as Leptospira interrogans. Serovars Pomona and Butembo were detected using MAT and the highest titre was 200 for serovar Pomona. Epidemiological aspects of the findings are discussed.
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Base de datos:
LILACS
Asunto principal:
Ciervos
/
Leptospira interrogans
/
Leptospirosis
/
Anticuerpos Antibacterianos
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Límite:
Animales
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2011
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Embrapa Gado de Corte/BR
/
Embrapa Pantanal/BR
/
Independent Consultant on Veterinary/BR
/
Universidade Federal do Mato Grosso do Sul/BR
/
Universidade de Brasília/BR
/
Universidade de São Paulo/BR