Amylase production by solid-state fermentation of agro-industrial wastes using Bacillus sp
Braz. j. microbiol
; 42(4): 1334-1342, Oct.-Dec. 2011. ilus, tab
Article
en En
| LILACS
| ID: lil-614593
Biblioteca responsable:
BR32.1
ABSTRACT
Solid state fermentation was carried out using various agro- industrial wastes with the best amylase producing strain isolated from soil. Different physicochemical conditions were varied for maximum enzyme production. The strain produced about 5400 units/g of amylase at 13 moisture content, 20 percent inoculum, after 72 h of incubation with Mustard Oil seed cake as the substrate. The optimum temperature and pH of the enzyme activity were found to be 50ºC and 6 respectively. The enzyme was found to be thermostable at 70ºC for about 2 h without any salt. It showed stability at pH range 5-7. The metal ions as Na+, Ca++, Mg++ and Co++ enhanced the enzyme activity.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Suelo
/
Condición del Suelo
/
Activación Enzimática
/
Agroindustria
/
Fermentación
/
Residuos de Alimentos
/
Amilasas
Idioma:
En
Revista:
Braz. j. microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2011
Tipo del documento:
Article
País de afiliación:
India
Pais de publicación:
Brasil