A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia
Mem. Inst. Oswaldo Cruz
; 108(3): 390-391, maio 2013.
Article
en En
| LILACS
| ID: lil-676966
Biblioteca responsable:
BR1.1
ABSTRACT
Stenotrophomonas maltophilia is a multidrug-resistant nosocomial pathogen that is difficult to identify unequivocally using current methods. Accordingly, because the presence of this microorganism in a patient may directly determine the antimicrobial treatment, conventional polymerase chain reaction (PCR) and real-time PCR assays targeting 23S rRNA were developed for the specific identification of S. maltophilia. The PCR protocol showed high specificity when tested against other species of Stenotrophomonas, non-fermentative Gram-negative bacilli and 100 clinical isolates of S. maltophilia previously identified using the Vitek system.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Reacción en Cadena de la Polimerasa
/
Stenotrophomonas maltophilia
Tipo de estudio:
Guideline
Límite:
Humans
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Año:
2013
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Brasil