Vagotomy ameliorates islet morphofunction and body metabolic homeostasis in MSG-obese rats
Braz. j. med. biol. res
; 48(5): 447-457, 05/2015. tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-744369
Biblioteca responsable:
BR1.1
ABSTRACT
The parasympathetic nervous system is important for β-cell secretion and mass regulation. Here, we characterized involvement of the vagus nerve in pancreatic β-cell morphofunctional regulation and body nutrient homeostasis in 90-day-old monosodium glutamate (MSG)-obese rats. Male newborn Wistar rats received MSG (4 g/kg body weight) or saline [control (CTL) group] during the first 5 days of life. At 30 days of age, both groups of rats were submitted to sham-surgery (CTL and MSG groups) or subdiaphragmatic vagotomy (Cvag and Mvag groups). The 90-day-old MSG rats presented obesity, hyperinsulinemia, insulin resistance, and hypertriglyceridemia. Their pancreatic islets hypersecreted insulin in response to glucose but did not increase insulin release upon carbachol (Cch) stimulus, despite a higher intracellular Ca2+ mobilization. Furthermore, while the pancreas weight was 34% lower in MSG rats, no alteration in islet and β-cell mass was observed. However, in the MSG pancreas, increases of 51% and 55% were observed in the total islet and β-cell area/pancreas section, respectively. Also, the β-cell number per β-cell area was 19% higher in MSG rat pancreas than in CTL pancreas. Vagotomy prevented obesity, reducing 25% of body fat stores and ameliorated glucose homeostasis in Mvag rats. Mvag islets demonstrated partially reduced insulin secretion in response to 11.1 mM glucose and presented normalization of Cch-induced Ca2+ mobilization and insulin release. All morphometric parameters were similar among Mvag and CTL rat pancreases. Therefore, the higher insulin release in MSG rats was associated with greater β-cell/islet numbers and not due to hypertrophy. Vagotomy improved whole body nutrient homeostasis and endocrine pancreatic morphofunction in Mvag rats.
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Contexto en salud:
ODS3 - Meta 3A Fortalecer la implementación de la estructura de la Convención de la Organización Mundial de la Salud para el Control del Tabaco
/
ODS3 - Salud y Bienestar
/
ODS3 - Meta 3.5 Prevención y tratamiento del consumo de sustancias psicoactivas
Problema de salud:
Educación, Comunicación, Capacitación y Conciencia Pública
/
Meta 3.5: Prevención y tratamiento del consumo de sustancias psicoactivas
/
Abuso de Opióides
Base de datos:
LILACS
Asunto principal:
Fumar
/
Conocimientos, Actitudes y Práctica en Salud
/
Cese del Hábito de Fumar
/
Trastornos Relacionados con Sustancias
Límite:
Adulto
/
Femenino
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Braz. j. med. biol. res
Asunto de la revista:
Biologia
/
Medicina
Año:
2015
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Estadual do Oeste do Paraná/BR