Normal mesenteric lymph ameliorates lipopolysaccharide challenge-induced spleen injury
Acta cir. bras
; Acta cir. bras;30(9): 604-610, Sep. 2015. ilus
Article
en En
| LILACS
| ID: lil-761496
Biblioteca responsable:
BR1.1
ABSTRACT
PURPOSE:
This study was conducted to investigate the effect of normal mesenteric lymph (NML) from mice on the spleen injury induced by lipopolysaccharide (LPS) challenge.METHODS:
Mice in the LPS and LPS+NML groups received an intraperitoneal injection of LPS (35 mg/kg) and kept for 6 h.. The mice in the LPS+NML group received NML treatment at 1 h after LPS injection. Afterward, the splenic morphology, the levels of lipopolysaccharide-binding protein (LBP), cluster of differentiation 14 (CD14), phosphorylation mitogen-activated protein kinases (MAPKs), and inflammatory mediators in splenic tissue were investigated.RESULTS:
LPS injection induced spleen injury, increased the levels of LBP, CD14, tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), and interferon γ (IFN-γ), and decreased the IL-4 content in the spleen. By contrast, NML treatment reversed these changes. Meanwhile, the LPS challenge decreased the phosphorylation levels of p38 MAPK, extracellular regulated protein kinases 1/2, and c-Jun N-terminal kinase (JNK). Moreover, the phosphorylation levels of p38 MAPK and JNK were further decreased by the NML administration.CONCLUSION:
rRdThe normal mesenteric lymph treatment alleviated lipopolysaccharide induced spleen injury by attenuating LPS sensitization and production of TNF-α, IL-6, and IFN-γ.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Enfermedades del Bazo
/
Lipopolisacáridos
/
Ganglios Linfáticos
/
Mesenterio
Tipo de estudio:
Evaluation_studies
Límite:
Animals
Idioma:
En
Revista:
Acta cir. bras
Asunto de la revista:
Cirurgia Geral
/
Procedimentos Cir£rgicos Operat¢rios
Año:
2015
Tipo del documento:
Article
/
Project document
País de afiliación:
China
Pais de publicación:
Brasil