Antibodies for Rickettsia spp. in patients with negative serology for dengue virus, leptospirosis, and meningococcal disease in municipalities of São Paulo State, Brazil
Rev. Soc. Bras. Med. Trop
; 49(5): 567-571, Sept.-Oct. 2016. tab
Article
en En
| LILACS, SES-SP
| ID: lil-798124
Biblioteca responsable:
BR1.1
ABSTRACT
Abstract INTRODUCTION Brazilian spotted fever is an infectious disease with a high mortality rate if not treated early. Differential diagnosis is difficult, as the first clinical signs are non-specific and can be confused with other diseases. The aim of the study was to investigate evidence of infection with Rickettsia rickettsii and Rickettsia parkeri in negative sera samples, collected in 2014, from patients with suspected leptospirosis, dengue fever, and meningococcal disease in Atibaia and Bragança Paulista municipalities of the State of São Paulo. METHODS The samples stored at the Institute Adolfo Lutz in Campinas were tested using an indirect immunofluorescence assay (IFA) with IgG and IgM against R. rickettsii and R. parkeri. Real-time polymerase chain reaction (PCR) testing was performed for the sera samples of patients who died (n = 3), those with initial suspicion of meningococcal disease (n = 6), and those with positive IFA results. RESULTS Of 258 samples from Bragança Paulista, 4 (1.6%) were positive, with IgG titers of 164 and 1128 against R. rickettsii and R. parkeri, respectively. Of 155 samples from Atibaia, 2 (1.3%) were positive, with IgG titers of 164 and 1128 against R. rickettsii and R. parkeri, respectively. No sample showed positive PCR results. CONCLUSIONS This serological investigation suggests there is evidence of exposure to Rickettsia spp. in residents of areas that have environmental conditions favorable to the spread of bacteria, in which Brazilian spotted fever incidence was not previously confirmed.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
/
SES-SP
Asunto principal:
Rickettsia
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Infecciones por Rickettsia
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Inmunoglobulina G
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Inmunoglobulina M
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Anticuerpos Antibacterianos
Tipo de estudio:
Diagnostic_studies
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Prevalence_studies
/
Risk_factors_studies
Límite:
Adult
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Female
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Humans
/
Male
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Rev. Soc. Bras. Med. Trop
Asunto de la revista:
MEDICINA TROPICAL
Año:
2016
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Brasil