Optical recording of membrane potential on isolated spiral ganglion cells of newborn mice using a voltage-sensitive dye.
Acta Otolaryngol Suppl
; 539: 34-9, 1998.
Article
en En
| MEDLINE
| ID: mdl-10095858
ABSTRACT
Optical imaging methods make monitoring of the membrane potential feasible. With this technique, one can observe different optical signals depending on optical properties, in response to membrane potential, using voltage-sensitive dyes. We used the multiple-site optical imaging system to investigate membrane potentials of the isolated, cultured spiral ganglion cells (SCGs) from newborn mice. We used a voltage-sensitive absorption dye. With high potassium (150 mM K+) exposure, the absorbency of SGCs stained with voltage-sensitive dye increased temporarily under a 700-nm interference filter at approximately 0.3%. This detection of the depolarization of SGCs using an optical recording technique with a voltage-sensitive dye is important because it shows that the simultaneous measurement of activity in a variety of regions is possible, as is the exploring of the intercellular signalling pathway.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ganglio Espiral de la Cóclea
/
Potenciales de la Membrana
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Acta Otolaryngol Suppl
Año:
1998
Tipo del documento:
Article
País de afiliación:
Japón