Transgenic mice ubiquitously expressing human placental alkaline phosphatase (PLAP): an additional reporter gene for use in tandem with beta-galactosidase (lacZ).
Int J Dev Biol
; 43(1): 85-90, 1999 Jan.
Article
en En
| MEDLINE
| ID: mdl-10213086
ABSTRACT
A fundamental keystone of developmental biology has been the growing use of reporter genes in model transgenic systems. Their use has greatly facilitated investigations of cell lineage and cell fate in addition to aiding experiments aimed at determining patterns of gene expression, gene interaction and gene regulation. Through construction of transgenic mice, ubiquitously expressing human placental alkaline phosphatase (PLAP), we demonstrate the suitability of PLAP as a reporter gene for use in conjunction with, or as an alternative to, beta-galactosidase (lacZ). Our findings demonstrate that over-expression of PLAP has no adverse effects on mouse development or viability, despite a widespread pattern of expression. This technology provides a simple yet effective mechanism based on eukaryotic reporter gene technology to facilitate the identification of transgenic cells within complex in vivo systems.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ratones Transgénicos
/
Proteínas
/
Beta-Galactosidasa
/
Genes Reporteros
/
Biología Molecular
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Int J Dev Biol
Asunto de la revista:
BIOLOGIA
/
EMBRIOLOGIA
Año:
1999
Tipo del documento:
Article
País de afiliación:
Reino Unido