Gas chromatographic assay of cellular fatty acids and alcohols for the identification of Mycobacterium species.
New Microbiol
; 22(2): 151-4, 1999 Apr.
Article
en En
| MEDLINE
| ID: mdl-10322614
ABSTRACT
Ten mycobacterial species obtained from 141 cultures isolated from clinical specimens were studied. The cultures were grown on solid medium and then analysed-after saponification, methylation, extraction with organic solvent and washing of the organic phase--by capillary gas-liquid chromatography for fatty acid and secondary alcohol composition. The absence of secondary alcohols was characteristic of M. genavense, M. tuberculosis and the following Mycobacterium species with specific branched-chain fatty acids allowing their direct identification M. gordonae, M. kansasii and M. marinum. The presence of secondary alcohols was characteristic of M. avium, M. phlei, M. scrofulaceum, M. terrae and M. xenopi. In the case of M. xenopi direct identification was made possible by the presence of a specific alcohol.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cromatografía de Gases
/
Técnicas de Tipificación Bacteriana
/
Alcoholes
/
Ácidos Grasos
/
Mycobacterium
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
New Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
1999
Tipo del documento:
Article
País de afiliación:
Italia