A mechanism of resistance to HIV-1 entry: inefficient interactions of CXCR4 with CD4 and gp120 in macrophages.
Virology
; 259(1): 1-6, 1999 Jun 20.
Article
en En
| MEDLINE
| ID: mdl-10364484
To test the hypothesis that inefficient interactions of CXCR4 with CD4 and gp120 could affect HIV-1 entry, we incubated macrophages, monocytes, and lymphocytes with gp120 and coimmunoprecipitated CD4 by using anti-CXCR4 antibodies. CD4 was efficiently coimmunoprecipitated in lymphocytes and monocytes but not in macrophages. Overexpression of CD4 in macrophages resulted in detection of CD4-CXCR4 and gp120-CD4-CXCR4 complexes in parallel with the restoration of macrophage fusion susceptibility. These results suggest a mechanism of resistance to entry of some X4 HIV-1 strains into macrophages and a method for dissection of the initial stages of HIV entry.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Antígenos CD4
/
Proteína gp120 de Envoltorio del VIH
/
Síndrome de Inmunodeficiencia Adquirida
/
VIH-1
/
Receptores CXCR4
/
Macrófagos
Límite:
Humans
Idioma:
En
Revista:
Virology
Año:
1999
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos