Circadian synthesis of a nuclear-encoded chloroplast glyceraldehyde-3-phosphate dehydrogenase in the dinoflagellate Gonyaulax polyedra is translationally controlled.
Biochemistry
; 38(24): 7689-95, 1999 Jun 15.
Article
en En
| MEDLINE
| ID: mdl-10387008
ABSTRACT
The circadian clock has previously been shown to restrict synthesis of several proteins in the dinoflagellate Gonyaulax polyedra to only a few hours each day. We have identified one of these proteins as glyceraldehyde-3-phosphate dehydrogenase. Two nuclear genes encoding the enzyme have been cloned, one corresponding to a cytoplasmic isoform and the other to a plastid targeted protein. On the basis of protein microsequence data, we conclude that the synthesis of the plastid isoform is clock-regulated. This regulation is not related to mRNA levels, which remain constant throughout the cycle, suggesting a translational control mechanism, in contrast to the transcriptional regulation of GAPDH that has been demonstrated in Neurospora. Although the rhythm of synthesis has a high amplitude, the abundance and activity rhythms are greatly attenuated, which is attributed to the long half-life of the protein.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Biosíntesis de Proteínas
/
Dinoflagelados
/
Núcleo Celular
/
Cloroplastos
/
Ritmo Circadiano
/
Gliceraldehído-3-Fosfato Deshidrogenasas
Límite:
Animals
Idioma:
En
Revista:
Biochemistry
Año:
1999
Tipo del documento:
Article
País de afiliación:
Estados Unidos