Some aspects of oligoribonucleotides synthesis via the H-phosphonate approach.

ABSTRACT

This review gives a short account of selected aspects of oligoribonucleotide synthesis via the H-phosphonate method. It includes (i) recent methods for the preparation of suitably protected ribonucleoside 3'-H-phosphonates (the phosphonylation step), (ii) some chemical and stereochemical features of the formation of H-phosphonate internucleosidic linkages, and (iii) stereoselective synthesis of oligoribonucleoside phosphorothioates using chemo-enzymatic approach.