Distribution pattern of a flavonoid extract in the gastrointestinal lumen and wall of rats.

ABSTRACT

Purified flavonoid extract from illuminated parsley (Petroselinum crispum (Mill.) Nym.) cell culture was administered by gavage to Wistar rats. The dose corresponded to 6.9 mg flavonoids on aglycone base/kg body mass. Segments of the gastrointestinal wall from stomach to colon, their luminal contents, and liver and kidneys were collected at time intervals between 1 and 12 h and investigated by HPLC of the respective extracts for flavonoids. The spreading of the flavonoids was accompanied by partial deglycosylation that began already in the stomach where at first quercetin and later apigenin, chrysoeriol and isorhamnetin aglycones were detected. We got evidence of flavonoid absorption by the stomach that does not require the liberation of aglycones. Due to obvious differences in metabolization and absorption rates the composition and the content of flavonoids changes in the gastrointestinal segments and their contents with time. Flavonoids could be detected neither within the gastrointestinal lumen after 12 h nor in the kidneys at any time. But traces of flavonoids were found in the livers at 1.5 and 12 h.