Influence of plasma membrane depolarization on cAMP level in rat brain synaptosomes.
Neuroreport
; 10(8): 1763-5, 1999 Jun 03.
Article
en En
| MEDLINE
| ID: mdl-10501571
ABSTRACT
We studied the influence of plasma membrane depolarization on cAMP content in presynaptic nerve endings (synaptosomes) isolated from brain hemispheres (HS) and cerebellum (CS). Depolarization by elevated [K+]o decreased basal cAMP level in both types of synaptosomes; reduced cAMP content in HS and increased cAMP in CS in the presence of IBMX; and lowered forskolin-stimulated cAMP accumulation in both the HS and the CS. Similar results were obtained when depolarization was induced by veratrine or when [Ca2+]i was elevated by treatment of the synaptosomes with the ionophore A23187. In Ca2+-free media, depolarization was not able to affect the synaptosomal cAMP levels. These data suggest that in brain synaptosomes intracellular cAMP pathway is modulated by alterations in [Ca2+]i.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sinaptosomas
/
Encéfalo
/
AMP Cíclico
Límite:
Animals
Idioma:
En
Revista:
Neuroreport
Asunto de la revista:
NEUROLOGIA
Año:
1999
Tipo del documento:
Article