A generic protein purification method for protein complex characterization and proteome exploration.
Nat Biotechnol
; 17(10): 1030-2, 1999 Oct.
Article
en En
| MEDLINE
| ID: mdl-10504710
ABSTRACT
We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, activity, or function. Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been tested in yeast but should be applicable to other cells or organisms.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas
/
Proteoma
/
Métodos
Idioma:
En
Revista:
Nat Biotechnol
Asunto de la revista:
BIOTECNOLOGIA
Año:
1999
Tipo del documento:
Article
País de afiliación:
Alemania