A hemizygous short tandem repeat polymorphism 3' to the human phosphoglycerate kinase gene.
Mol Biol Rep
; 26(3): 159-65, 1999 Aug.
Article
en En
| MEDLINE
| ID: mdl-10532310
ABSTRACT
The human phosphoglycerate kinase (PGK) gene is located within Xq11-Xq13, a region implicated in genitourinary diseases including prostate cancer, androgen insensitivity, perineal hypospadias, and other genetic abnormalities. The PGK gene and the androgen receptor gene are in linkage disequilibrium. PGK has been mapped extensively for nuclease-sensitive sites, methylation sites, and flanking DNA sequences. A PGK-associated BstXI polymorphism has been used to determine clonality of neoplastic tissues. Using fluorescent PCR product analysis and DNA sequencing, we discovered that a short tandem repeat (STR) in the 3' flanking region of the PGK gene is polymorphic. Among 231 individuals, there were nine distinct alleles, including eight based on variations in the number of TATC repeats. The PGK STR demonstrated hemizygosity, consistent with its X-chromosomal location and with an absence of cross-hybridizing autosomal homologs. The polymorphic PGK STR shows promise for rapid investigation of neoplastic clonality, for personal identification, and for studies of inherited predisposition to urologic disorders.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fosfoglicerato Quinasa
/
Polimorfismo Genético
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Cromosoma X
/
Secuencias Repetidas en Tándem
Tipo de estudio:
Observational_studies
/
Prevalence_studies
/
Risk_factors_studies
Límite:
Female
/
Humans
/
Male
Idioma:
En
Revista:
Mol Biol Rep
Año:
1999
Tipo del documento:
Article
País de afiliación:
Estados Unidos