C/EBP regulates the promoter of the eosinophil-derived neurotoxin/RNS2 gene in human eosinophilic cells.
J Leukoc Biol
; 66(4): 683-8, 1999 Oct.
Article
en En
| MEDLINE
| ID: mdl-10534126
ABSTRACT
The eosinophil-derived neurotoxin (EDN), a member of the mammalian ribonuclease family, is found in the large specific granules of human eosinophilic leukocytes. We have investigated the role of the C/EBP transcription factor family in the regulation of EDN promoter activity. Here we show that the C/EBP family is involved in intrinsic regulation of EDN promoter activity. We have identified a C/EBP binding site located at -124 in the proximal promoter of the EDN gene. Mutation of this C/EBP site results in a decrease of promoter activity in HL-60-eos cells as well as in eosinophils differentiated in vitro from CD34+ cells. Different C/EBP proteins are able to bind to the C/EBP site as shown by gel shift assay. Our results indicate the importance of the C/EBP family in the regulation of the EDN gene in eosinophils.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ribonucleasas
/
Proteínas Nucleares
/
Proteínas
/
Regiones Promotoras Genéticas
/
Proteínas de Unión al ADN
/
Eosinófilos
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Leukoc Biol
Año:
1999
Tipo del documento:
Article
País de afiliación:
Países Bajos