Convenient synthesis of lactuloselysine and its use for LC-MS analysis in milk-like model systems.
J Agric Food Chem
; 47(11): 4700-6, 1999 Nov.
Article
en En
| MEDLINE
| ID: mdl-10552875
The synthesis of the Amadori product lactuloselysine [N(epsilon)-(1-deoxy-D-lactulosyl-1)-L-lysine] was obtained starting from FMOC-lysine-OH (N(alpha)-9-fluorenylmethoxy-carbonyl-N(epsilon)H(2)-L-lysine-OH) and lactose. Compound identity was confirmed by MALDI-ToF, electrospray, and NMR analysis. A selective LC-MS procedure which allowed the detection of lactuloselysine up to 10 ng mL(-)(1) was set up and used to follow the formation of the compound in a lactose-lysine model system; quantification of this molecule after complete enzymatic hydrolysis of whey-proteins from milk samples was also performed.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Leche
/
Lactulosa
Límite:
Animals
Idioma:
En
Revista:
J Agric Food Chem
Año:
1999
Tipo del documento:
Article
País de afiliación:
Italia
Pais de publicación:
Estados Unidos