Sheathless preconcentration-capillary zone electrophoresis-mass spectrometry applied to peptide analysis.
J Am Soc Mass Spectrom
; 10(12): 1271-8, 1999 Dec.
Article
en En
| MEDLINE
| ID: mdl-10584328
ABSTRACT
A reliable capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry system has been developed for the sensitive analysis and sequencing of peptides. The system comprises (1) a zero dead volume on-line sample preconcentration interface allowing over 100-fold increase in sample volume introduction and (2) a zero-dead volume, durable electrospray emitter yielding stable, low background electrospray signals, both proving not to compromize the electrophoretic performance. Sub-fmol sensitivities were obtained in applications to complex peptide samples derived from tryptic digestion of proteins and naturally processed major histocompatibility complex class I associated peptides.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Péptidos
Idioma:
En
Revista:
J Am Soc Mass Spectrom
Año:
1999
Tipo del documento:
Article
País de afiliación:
Países Bajos