Synthetic phytanyl-chained glycolipid vesicle membrane as a novel matrix for functional reconstitution of cyanobacterial photosystem II complex.
Biochem Biophys Res Commun
; 265(3): 734-8, 1999 Nov 30.
Article
en En
| MEDLINE
| ID: mdl-10600489
ABSTRACT
The vesicles composed of synthetic phytanyl-chained glycolipid and natural sulfoquinovosyldiacylglycerol at 91 molar ratio were successfully applied to functional reconstitution of photosystem II complex (PS II) from a thermophilic cyanobacterium. The synthetic glycolipid employed was one of our model archaeal diether lipids, 1, 3-di-O-phytanyl-2-O-(beta-D-maltotriosyl)glycerol. The light-induced oxygen-evolving activity of PS II reconstituted in the glycolipid vesicles was approximately 6-fold higher than that reconstituted in several phosphatidylcholine vesicles. The present results reveal the first evidence that a well-designed synthetic glycolipid is effective for the functional reconstitution of complicated and labile membrane protein complexes, such as PS II.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Glucolípidos
/
Cianobacterias
/
Proteínas del Complejo del Centro de Reacción Fotosintética
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1999
Tipo del documento:
Article
País de afiliación:
Japón