Epstein-barr virus transformation: involvement of latent membrane protein 1-mediated activation of NF-kappaB.
Oncogene
; 18(49): 6959-64, 1999 Nov 22.
Article
en En
| MEDLINE
| ID: mdl-10602470
ABSTRACT
Epstein-Barr virus (EBV) transforms resting primary human B lymphocytes into indefinitely proliferating lymphoblastoid cell lines in vitro and is associated with several human malignancies in vivo. Recombinant EBV genetic analyses combined with in vitro B lymphocyte transformation assays demonstrate that latent infection membrane protein 1 (LMP1) is essential for EBV-mediated lymphocyte transformation. LMP1 has no intrinsic enzymatic activity but instead aggregates cellular proteins of the tumor necrosis factor receptor signaling pathway to activate transcription factor NF-kappaB. Mutants rendering LMP1 defective in these protein interactions are impaired in their abilities to activate NF-kappaB in reporter gene assays. Concordantly, EBV recombinants with LMP1 mutations that are compromised for NF-kappaB activation are impaired for growth transformation. Thus, EBV-mediated growth transformation is genetically and biochemically linked to LMP1-mediated activation of NF-kappaB.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Transformación Celular Viral
/
Proteínas de la Matriz Viral
/
FN-kappa B
/
Herpesvirus Humano 4
/
Proteínas I-kappa B
Límite:
Humans
Idioma:
En
Revista:
Oncogene
Asunto de la revista:
BIOLOGIA MOLECULAR
/
NEOPLASIAS
Año:
1999
Tipo del documento:
Article
País de afiliación:
Estados Unidos