Purification and characterization of lecithin:cholesterol acyltransferase.
Scand J Clin Lab Invest Suppl
; 150: 1-5, 1978.
Article
en En
| MEDLINE
| ID: mdl-106459
ABSTRACT
The purification of lecithincholesterol acyltransferase (LCAT] from human plasma is reported. Hydroxylapatite fractions were approximately 16,000 fold purified over the starting plasma and were free of high-density lipoprotein (HDL) and albumin. The enzyme showed one band on polyacrylamide gel electrophoresis, SDS-urea polyacrylamide gel electrophoresis, isoelectric focusing, and one arc in immunodiffusion against a goat antiserum preparation. It was determined to be a glycoprotein with a molecular weight of approximately 70,000 and a pI of 3.7--4.0.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fosfatidilcolina-Esterol O-Aciltransferasa
Límite:
Humans
Idioma:
En
Revista:
Scand J Clin Lab Invest Suppl
Año:
1978
Tipo del documento:
Article