Combinatorial gene expression using multiple episomal vectors.
Gene
; 243(1-2): 187-94, 2000 Feb 08.
Article
en En
| MEDLINE
| ID: mdl-10675627
ABSTRACT
Episomal vectors offer a powerful alternative to integrative recombination for transgene expression in mammalian cells. In this study, various combinations of G protein-coupled receptors (GPCRs) and the G protein subunit G(i2)alpha, were stably expressed from separate episomal vectors in 293-EBNA (293E) cells. Each episome did not adversely affect the others, as gauged by episomal copy number, steady-state mRNA levels and the presence of functional receptors and G protein. Cell lines expressing genes from multiple autonomously replicating vectors were stable just two weeks after transfection, and remained stable in continuous culture for at least 5months. Co-expression of supplementary G(i2)alpha with receptor amplifies the magnitude of signal transduction thereby permitting the development of more sensitive high throughput functional assays. Given these results, combinatorial transfection is the strategy of choice for generating stable cell lines expressing multiple genes for the study of signal-transduction pathways or the evaluation of receptor ligands.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plásmidos
/
Regulación de la Expresión Génica
/
Subunidades alfa de la Proteína de Unión al GTP Gi-Go
Idioma:
En
Revista:
Gene
Año:
2000
Tipo del documento:
Article
País de afiliación:
Estados Unidos