Identification of planorbids from Venezuela by polymerase chain reaction amplification and restriction fragment length polymorphism of internal transcriber spacer of the RNA ribosomal gene.
Mem Inst Oswaldo Cruz
; 95(2): 171-7, 2000.
Article
en En
| MEDLINE
| ID: mdl-10733735
ABSTRACT
Snails of the genus Biomphalaria from Venezuela were subjected to morphological assessment as well as polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis. Morphological identification was carried out by comparison of characters of the shell and the male and female reproductive apparatus. The PCR-RFLP involved amplification of the internal spacer region ITS1 and ITS2 of the RNA ribosomal gene and subsequent digestion of this fragment by the restriction enzymes DdeI, MnlI, HaeIII and MspI. The planorbids were compared with snails of the same species and others reported from Venezuela and present in Brazil, Cuba and Mexico. All the enzymes showed a specific profile for each species, that of DdeI being the clearest. The snails were identified as B. glabrata, B. prona and B. kuhniana.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Biomphalaria
/
Polimorfismo de Longitud del Fragmento de Restricción
/
ARN Ribosómico
/
Reacción en Cadena de la Polimerasa
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
País/Región como asunto:
America do sul
/
Venezuela
Idioma:
En
Revista:
Mem Inst Oswaldo Cruz
Año:
2000
Tipo del documento:
Article
País de afiliación:
Brasil