Inhibitors present in blood do not inhibit PCR from buccal cell preparations: case report.
In Vivo
; 13(6): 453-4, 1999.
Article
en En
| MEDLINE
| ID: mdl-10757036
ABSTRACT
BACKGROUND:
Hemochromatosis is a common disease that is characterized by high ferritin levels and/or high iron saturation and mutations in two alleles. MATERIAL ANDMETHODS:
Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) is often performed on DNA extracted from blood since blood yields high concentrations of DNA. However, inhibitors can cause PCR failure in DNA extracted from blood thus preventing a molecular diagnosis.RESULTS:
This report describes a case where multiple blood draws resulted in unamplifiable DNA. Subsequently, a buccal cell sample was collected and extracted. DNA extracted from the buccal cells yielded amplifiable DNA in contrast to DNA extracted from the patient's blood. In addition, the patient was identified as having a homozygous mutation for one allele of the hemochromatosis gene.CONCLUSION:
These results suggests that a buccal cell DNA extraction may be useful in cases where blood samples contain inhibitory substances for PCR.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polimorfismo de Longitud del Fragmento de Restricción
/
Proteínas Sanguíneas
/
Reacción en Cadena de la Polimerasa
/
Hemocromatosis
/
Boca
Tipo de estudio:
Prognostic_studies
Límite:
Female
/
Humans
/
Middle aged
Idioma:
En
Revista:
In Vivo
Asunto de la revista:
NEOPLASIAS
Año:
1999
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
GR
/
GRECIA
/
GREECE
/
GRÉCIA