Multiplex PCRs for identification of Escherichia coli virulence genes.
J Clin Microbiol
; 38(5): 2001-4, 2000 May.
Article
en En
| MEDLINE
| ID: mdl-10790141
ABSTRACT
PCRs were developed to detect 11 Escherichia coli virulence genes. Primers amplified the respective genes without cross-reaction with other genes. Specificity was maintained in multiplex reactions; excellent amplification of target genes was possible with a minimum of four multiplex reactions. These reactions successfully identified genes in E. coli from the feces of four dogs.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Reacción en Cadena de la Polimerasa
/
Escherichia coli
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Clin Microbiol
Año:
2000
Tipo del documento:
Article
País de afiliación:
Australia