Hyaluronan oligosaccharides perturb cartilage matrix homeostasis and induce chondrocytic chondrolysis.
Arthritis Rheum
; 43(5): 1165-74, 2000 May.
Article
en En
| MEDLINE
| ID: mdl-10817571
ABSTRACT
OBJECTIVE:
To determine the role of CD44-mediated matrix assembly in maintaining cartilage homeostasis.METHODS:
Articular cartilage tissue slices as well as isolated chondrocytes were treated with hyaluronan (HA) hexasaccharides. Tissues and cells were processed for histology, immunohistochemistry, colorimetric assay, reverse transcriptase-polymerase chain reaction, and zymography.RESULTS:
HA oligosaccharides induced a dose-dependent state of chondrocytic chondrolysis, including near-total loss of stainable proteoglycan-rich matrix, activation of gelatinolytic activity, and exhibition of the NITEGE epitope. However, HA oligosaccharides also induced an increase of proteoglycan synthesis, including elevation of aggrecan and HA synthase 2 messenger RNA.CONCLUSION:
Uncoupling chondrocytes from the matrix results in deleterious changes in matrix structure and modifications in chondrocyte metabolism. The close interaction of chondrocytes with their matrix and their ability to "sense" changes in receptor occupancy are needed for maintenance of cartilage homeostasis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oligosacáridos
/
Cartílago
/
Cartílago Articular
/
Proteínas de la Matriz Extracelular
/
Ácido Hialurónico
Límite:
Humans
/
Male
Idioma:
En
Revista:
Arthritis Rheum
Año:
2000
Tipo del documento:
Article
País de afiliación:
Estados Unidos