Mechanism of ATP-dependent promoter melting by transcription factor IIH.
Science
; 288(5470): 1418-22, 2000 May 26.
Article
en En
| MEDLINE
| ID: mdl-10827951
ABSTRACT
We show that transcription factor IIH ERCC3 subunit, the DNA helicase responsible for adenosine triphosphate (ATP)-dependent promoter melting during transcription initiation, does not interact with the promoter region that undergoes melting but instead interacts with DNA downstream of this region. We show further that promoter melting does not change protein-DNA interactions upstream of the region that undergoes melting but does change interactions within and downstream of this region. Our results rule out the proposal that IIH functions in promoter melting through a conventional DNA-helicase mechanism. We propose that IIH functions as a molecular wrench rotating downstream DNA relative to fixed upstream protein-DNA interactions, thereby generating torque on, and melting, the intervening DNA.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
/
ADN
/
Adenosina Trifosfato
/
Regiones Promotoras Genéticas
/
Factores de Transcripción TFII
/
Proteínas de Unión al ADN
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Science
Año:
2000
Tipo del documento:
Article
País de afiliación:
Estados Unidos