Protein synthesis in Escherichia coli at 4 degrees C.
Electrophoresis
; 21(8): 1625-9, 2000 May.
Article
en En
| MEDLINE
| ID: mdl-10832897
ABSTRACT
Two-dimensional electrophoresis technology was used to investigate protein synthesis by the mesophilic bacterium Escherichia coli at low temperature. It was confirmed that protein synthesis in E. coli decreased strongly after a temperature downshift from 37 to 4 degrees C. After incubation for 150 min at 4 degrees C, however, the number of synthesized proteins represented 60% of the overall polypeptide number observed at 37 degrees C. Furthermore, the analysis of autoradiograms revealed the overexpression of 69 proteins by shocked bacteria, showing that the number of cold-induced proteins has been significantly underestimated so far.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Escherichia coli
Idioma:
En
Revista:
Electrophoresis
Año:
2000
Tipo del documento:
Article
País de afiliación:
Francia