Isolation and identification of bluetongue virus.
J Virol Methods
; 87(1-2): 13-23, 2000 Jun.
Article
en En
| MEDLINE
| ID: mdl-10856748
ABSTRACT
Bluetongue virus (BTV) is an arthropod-borne orbivirus that infects sheep, wild ruminants and occasionally cattle. Detection and specific identification of BTV is a multistep process. The first step involves the isolation of the virus from the animal's blood or other tissues, followed by inoculation of embryonating chicken eggs (ECE). After the virus has been amplified in ECE, it is passaged into BHK-21 cell culture for subsequent replication and identification. The virus is then amplified further and identified in microtiter plates by the immunoperoxidase assay using a group specific monoclonal antibody. Finally, the viral isolate is typed by a virus neutralization test.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Lengua Azul
/
Virus de la Lengua Azul
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Virol Methods
Año:
2000
Tipo del documento:
Article
País de afiliación:
Canadá