VMAT-Mediated changes in quantal size and vesicular volume.
J Neurosci
; 20(14): 5276-82, 2000 Jul 15.
Article
en En
| MEDLINE
| ID: mdl-10884311
ABSTRACT
It has been well established that the volume of secretory vesicles can be modulated. However, we present the first data demonstrating that the amount of transmitter in a vesicle can regulate its volume. Amperometry and transmission electron microscopy have been used to determine that l-3,4-dihydroxyphenylalanine and reserpine increase and decrease, respectively, the volume of single pheochromocytoma cell vesicles as well as their catecholamine content. Because changes in vesicular catecholamine content are tracked by changes in vesicle volume, our results indicate that when quantal size is altered via the vesicular monoamine transporter the concentration of catecholamines within the vesicles remains relatively constant. This previously unidentified cellular response provides new insight into how catecholamines can be packaged in and released from secretory vesicles.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de Transporte de Membrana
/
Neuropéptidos
/
Glicoproteínas de Membrana
/
Orgánulos
Límite:
Animals
Idioma:
En
Revista:
J Neurosci
Año:
2000
Tipo del documento:
Article
País de afiliación:
Estados Unidos