Bradykinin stimulates eotaxin production by a human lung fibroblast cell line.
J Allergy Clin Immunol
; 106(1 Pt 1): 117-23, 2000 Jul.
Article
en En
| MEDLINE
| ID: mdl-10887314
ABSTRACT
BACKGROUND:
Bradykinin, a potent inflammatory peptide, is increased in the airways of allergic patients. Accompanying the elevated bradykinin levels are increases in both eosinophils and fibroblasts. Eotaxin, a potent eosinophil-specific chemotactic factor, is released by fibroblasts and increased in the lower respiratory tract of allergic patients.OBJECTIVE:
We sought to test the hypothesis that lung fibro-blasts release eotaxin in response to bradykinin.METHODS:
The potential of bradykinin to induce the release of eotaxin from the human lung fibroblast cell line HFL-1 was tested by cell culture and evaluation of the culture supernatant fluids and RNA for immunoreactive eotaxin and eotaxin messenger RNA.RESULTS:
HFL-1 cells released eotaxin constitutively without stimulation, but bradykinin stimulated eotaxin release in a dose- and time-dependent manner and resulted in augmented expression of eotaxin messenger RNA. The release of eotaxin was sensitive to the action of glucocorticoids. Eosinophil chemotactic activity by HFL-1 supernatant fluids was inhibited by anti-human eotaxin-neutralizing antibody. Consistent with these results, inhibitors of bradykinin B2 receptors, but not bradykinin B1 receptors, inhibited bradykinin-induced eotaxin release.CONCLUSION:
These data demonstrate that bradykinin may stimulate lung fibroblasts to release eotaxin and suggest the potential for this mechanism to be important in modulation of lung inflammation.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bradiquinina
/
Factores Quimiotácticos Eosinófilos
/
Citocinas
/
Quimiocinas CC
/
Pulmón
Límite:
Humans
Idioma:
En
Revista:
J Allergy Clin Immunol
Año:
2000
Tipo del documento:
Article
País de afiliación:
Estados Unidos