Early onset of CD8 transgene expression inhibits the transition from DN3 to DP thymocytes.
J Immunol
; 165(3): 1236-42, 2000 Aug 01.
Article
en En
| MEDLINE
| ID: mdl-10903721
ABSTRACT
In this paper we show that the effects of transgenic coreceptor expression on thymocyte development depend on the onset of transgene expression. Thus, a CD8 transgene expressed on CD44+CD25+ (DN2) and CD44-CD25+ (DN3) cells causes a partial block at the stage when TCRbeta selection takes place and diminishes expansion at the subsequent developmental stages, resulting in increased DN3 and markedly reduced double-positive (DP) thymocyte numbers. This effect is evident on a polyclonal TCR repertoire as well as in TCR-transgenic mice (F5). By contrast, a CD8 transgene that leads to the same degree of overexpression on DP thymocytes, but is not expressed on double-negative subsets, has no effect on thymus size or composition. Therefore, the reduction of DP thymocyte numbers in CD8 TCRtg mice can be attributed to interferences at early developmental stages rather than to increased negative selection of DP cells.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Timo
/
Regulación de la Expresión Génica
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Subgrupos de Linfocitos T
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Receptores de Antígenos de Linfocitos T alfa-beta
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Antígenos CD8
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Transgenes
Límite:
Animals
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Humans
Idioma:
En
Revista:
J Immunol
Año:
2000
Tipo del documento:
Article
País de afiliación:
Reino Unido