Quantitative method for biomarkers of collagen degradation using liquid chromatography tandem mass spectrometry.
Anal Biochem
; 283(1): 71-6, 2000 Jul 15.
Article
en En
| MEDLINE
| ID: mdl-10929810
ABSTRACT
Preclinical efficacy testing commonly involves studies that require considerable resources and time. One valuable tool in this endeavor is the characterization of relevant biomarkers. A method has been developed for the simultaneous determination of collagen biomarker candidates as an instrument in screening compounds for efficacy. Two potential candidates, the 3-hydroxypyridinium crosslinks pyridinoline and deoxypyridinoline, were selected for analysis in collagen degradation models. Tissue or urine samples were collected, prepared, and quantitated for the biomarkers using spiked calibration curves and liquid chromatography tandem mass spectrometry. The development of a quick and simple assay method would allow us to increase the chances for success in efficacy screening by eliminating compounds with poor biomarker profiles. The method proposed here appears to be more selective, convenient, precise (generally <10% RSD), accurate (generally <10% RE), and sensitive relative to previously established methodology.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Biomarcadores
/
Colágeno
/
Cromatografía de Gases y Espectrometría de Masas
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Anal Biochem
Año:
2000
Tipo del documento:
Article
País de afiliación:
Estados Unidos