Steric bulk at cycloartenol synthase position 481 influences cyclization and deprotonation.
Org Lett
; 2(15): 2261-3, 2000 Jul 27.
Article
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| MEDLINE
| ID: mdl-10930258
ABSTRACT
Cycloartenol synthase converts oxidosqualene to the pentacyclic sterol precursor cycloartenol. An Arabidopsis thaliana cycloartenol synthase Ile481Val mutant was previously shown to produce lanosterol and parkeol in addition to its native product cycloartenol. Experiments are described here to construct Phe, Leu, Ala, and Gly mutants at position 481 and to determine their cyclization product profiles. The Phe mutant was inactive, and the Leu mutant produced cycloartenol and parkeol. The Ala and Gly mutants formed lanosterol, cycloartenol, parkeol, achilleol A, and camelliol C. Monocycles comprise most of the Gly mutant product, showing that an alternate cyclization route can be made the major pathway by a single nonpolar mutation.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Arabidopsis
/
Transferasas Intramoleculares
Idioma:
En
Revista:
Org Lett
Asunto de la revista:
BIOQUIMICA
Año:
2000
Tipo del documento:
Article
País de afiliación:
Estados Unidos