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Stationary-phase variation due to transposition of novel insertion elements in Xanthomonas oryzae pv. oryzae.
Rajeshwari, R; Sonti, R V.
Afiliación
  • Rajeshwari R; Centre for Cellular and Molecular Biology, Hyderabad 500 007, India.
J Bacteriol ; 182(17): 4797-802, 2000 Sep.
Article en En | MEDLINE | ID: mdl-10940020
Xanthomonas oryzae pv. oryzae causes bacterial leaf blight, a serious disease of rice. Spontaneous mutants which are deficient for virulence and extracellular polysaccharide (Eps) production accumulate in large numbers in stationary-phase cultures of this bacterium, a phenomenon which we have called stationary-phase variation. A clone (pSD1) carrying the Eps biosynthetic gene (gum) cluster of X. oryzae pv. oryzae restored Eps production and virulence to several spv (for stationary-phase variation) mutants. Data from localized recombination analysis, Southern hybridization, PCR amplification, and sequence analysis showed that the mutations are due to insertion of either one of two novel endogenous insertion sequence (IS) elements, namely, ISXo1 and ISXo2, into gumM, the last gene of the gum gene cluster. The results of Southern analysis indicate the presence of multiple copies of both IS elements in the genome of X. oryzae pv. oryzae. These results demonstrate the role of IS elements in stationary-phase variation in X. oryzae pv. oryzae.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos Bacterianos / Variación Genética / Xanthomonas / ADN Bacteriano / Elementos Transponibles de ADN Idioma: En Revista: J Bacteriol Año: 2000 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos Bacterianos / Variación Genética / Xanthomonas / ADN Bacteriano / Elementos Transponibles de ADN Idioma: En Revista: J Bacteriol Año: 2000 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos