Membrane permeabilization induced by discodermin A, a novel marine bioactive peptide.
Toxicon
; 39(2-3): 259-64, 2001.
Article
en En
| MEDLINE
| ID: mdl-10978743
ABSTRACT
The effects of discodermin A (DC-A), a novel marine bioactive peptide extracted from sea sponge Discodermia kiiensis, on the vascular smooth muscle cells and tissues were examined. Analysis with a confocal laser microscope showed that DC-A (0.1-30 microM) permeabilized the plasma membrane of A10 cells to the non-permeable fluorescent agents, ethidium homodimer-1 (MW = 857) and calcein (MW = 623), in a concentration-dependent manner. In the vascular tissue treated with 30 microM DC-A, addition of a micromolar concentration of Ca(2+) evoked a sustained contraction in the presence of ATP, suggesting that DC-A increased the permeability of the membrane to Ca(2+) and ATP. DC-A at higher concentrations (30 microM) significantly increased the leakage of lactate dehydrogenase (140 kD) from the vascular tissue. These results suggest that DC-A has a permeabilizing effect on the plasma membrane possibly by interacting with plasma membrane phospholipids with its six successive hydrophobic amino acid residues at N-terminal.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Permeabilidad de la Membrana Celular
/
Péptidos Catiónicos Antimicrobianos
/
Proteínas Anfibias
/
Músculo Liso Vascular
Límite:
Animals
Idioma:
En
Revista:
Toxicon
Año:
2001
Tipo del documento:
Article
País de afiliación:
Japón