Single injection of insulin delays the recurrence of diabetes in syngeneic islet-transplanted diabetic NOD mice.
Transplantation
; 70(6): 976-9, 2000 Sep 27.
Article
en En
| MEDLINE
| ID: mdl-11014652
ABSTRACT
BACKGROUND:
Insulin has been implicated in the pathogenesis of type 1 diabetes and oral administration of insulin has been shown to delay the onset of diabetes in NOD mice. In this study we determined whether a single footpad injection of insulin will protect syngeneic islet grafts from autoimmune destruction when placed under the kidney capsule of diabetic NOD mice.METHODS:
Five hundred islets were transplanted under the kidney capsule of diabetic female NOD mice in conjunction with a single footpad injection of either pork insulin in saline or mixed with incomplete Freund's adjuvant (IFA). Control groups received either IFA or saline alone.RESULTS:
Seven of 11 animals (63.6%) given insulin in IFA exhibit long-term graft survival (>75 days; mean +/- SEM >85.4+/-16.1) whereas only 3 of 12 animals (25.0%) in the IFA group had graft survival longer than 75 days (mean +/- SEM >41.9+/-12.8 days). In contrast, none of the animals that received insulin in saline (17.3+/-2.5 days) and saline only (16.1+2.0 days) exhibit prolonged graft survival.CONCLUSION:
These results suggest that a single footpad injection of insulin can protect the islet graft from immune attack in NOD mice.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Diabetes Mellitus
/
Insulina
Límite:
Animals
Idioma:
En
Revista:
Transplantation
Año:
2000
Tipo del documento:
Article
País de afiliación:
Canadá