Complementation of Myc-dependent cell proliferation by cDNA expression library screening.
Oncogene
; 19(42): 4828-31, 2000 Oct 05.
Article
en En
| MEDLINE
| ID: mdl-11039899
ABSTRACT
The targeted knockout of the c-myc gene from rat fibroblasts leads to a stable defect in cell proliferation. We used complex cDNA libraries expressed from retroviral vectors and an efficient sorting procedure to rapidly select for cDNAs that can restore the growth rate of c-myc deficient cells. All of the biologically active cDNAs contained either c-myc or N-myc, suggesting that no other cellular genes can effectively bypass the requirement for c-myc in fibroblast proliferation. This approach provides a powerful screening method for cell cycle changes in genetically defined systems.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
División Celular
/
Genes myc
/
Proteínas Proto-Oncogénicas c-myc
/
ADN Complementario
/
Genes cdc
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Oncogene
Asunto de la revista:
BIOLOGIA MOLECULAR
/
NEOPLASIAS
Año:
2000
Tipo del documento:
Article
País de afiliación:
Estados Unidos