Specific secretion of active single-chain Fv antibodies into the supernatants of Escherichia coli cultures by use of the hemolysin system.
Appl Environ Microbiol
; 66(11): 5024-9, 2000 Nov.
Article
en En
| MEDLINE
| ID: mdl-11055959
ABSTRACT
A simple method for the nontoxic, specific, and efficient secretion of active single-chain Fv antibodies (scFvs) into the supernatants of Escherichia coli cultures is reported. The method is based on the well-characterized hemolysin transport system (Hly) of E. coli that specifically secretes the target protein from the bacterial cytoplasm into the extracellular medium without a periplasmic intermediate. The culture media that accumulate these Hly-secreted scFv's can be used in a variety of immunoassays without purification. In addition, these culture supernatants are stable over long periods of time and can be handled basically as immune sera.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Región Variable de Inmunoglobulina
/
Fragmentos de Inmunoglobulinas
/
Proteínas de Escherichia coli
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Escherichia coli
/
Vectores Genéticos
/
Proteínas Hemolisinas
Tipo de estudio:
Evaluation_studies
Idioma:
En
Revista:
Appl Environ Microbiol
Año:
2000
Tipo del documento:
Article
País de afiliación:
España