An ELISA for the detection of Bacillus subtilis L-form bacteria confirms their symbiosis in strawberry.
Lett Appl Microbiol
; 31(5): 390-4, 2000 Nov.
Article
en En
| MEDLINE
| ID: mdl-11069643
ABSTRACT
AIMS:
To develop an ELISA for the detection of antigens derived from stable Bacillus subtilis L-form bacteria and to detect these in plants injected with L-form bacteria. METHODS ANDRESULTS:
A sandwich ELISA was developed and its specificity was investigated using L-forms and cell-walled forms of B. subtilis, different Bacillus species and a range of bacteria isolated from glasshouse-grown strawberry plants. The detection limits of the ELISA were approximately 10(3) viable cells ml(-1) for L-forms compared with 10(7) viable cells ml(-1) for cell-walled forms. Results showed that L-forms survived and moved within strawberry tissues injected with L-form bacteria.CONCLUSION:
An ELISA that selectively detects B. subtilis L-form bacteria was developed and shown to confirm the presence of L-forms in plants. SIGNIFICANCE AND IMPACT OF THE STUDY This will be a valuable rapid method to further studies on L-form plant interactions.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Simbiosis
/
Bacillus subtilis
/
Ensayo de Inmunoadsorción Enzimática
/
Frutas
/
Formas L
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Lett Appl Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2000
Tipo del documento:
Article
País de afiliación:
Reino Unido