Telomeric repeat amplification, without shortening or lengthening of the telomerase products: a method to analyze the processivity of telomerase enzyme.
Nucleic Acids Res
; 29(2): E3, 2001 Jan 15.
Article
en En
| MEDLINE
| ID: mdl-11139633
ABSTRACT
The Telomeric Repeat Amplification Protocol (TRAP) and its modified versions (including ours, TP-TRAP) change the size and/or the ratio of the telomerase products in the amplification stage of the assay. Based on our recently published method we developed a new TRAP. This method ensures that the number of telomeric repeats present in the original telomerase products does not change on PCR amplification. The usefulness of the method was proved with amplification of chemically synthesized telomerase products and a newly designed telomerase substrate oligonucleotide. This is the first report in which the PCR products directly reflect the size distribution of telomerase products generated by the enzyme.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Secuencias Repetitivas de Ácidos Nucleicos
/
Procesamiento Proteico-Postraduccional
/
Telómero
/
Telomerasa
/
Técnicas de Amplificación de Ácido Nucleico
Límite:
Humans
Idioma:
En
Revista:
Nucleic Acids Res
Año:
2001
Tipo del documento:
Article
País de afiliación:
Hungria