Cell polarity and hyphal morphogenesis are controlled by multiple rho-protein modules in the filamentous ascomycete Ashbya gossypii.
Genetics
; 157(2): 601-10, 2001 Feb.
Article
en En
| MEDLINE
| ID: mdl-11156982
Polarized cell growth requires a polarized organization of the actin cytoskeleton. Small GTP-binding proteins of the Rho-family have been shown to be involved in the regulation of actin polarization as well as other processes. Hyphal growth in filamentous fungi represents an ideal model to investigate mechanisms involved in generating cell polarity and establishing polarized cell growth. Since a potential role of Rho-proteins has not been studied so far in filamentous fungi we isolated and characterized the Ashbya gossypii homologs of the Saccharomyces cerevisiae CDC42, CDC24, RHO1, and RHO3 genes. The AgCDC42 and AgCDC24 genes can both complement conditional mutations in the S. cerevisiae CDC42 and CDC24 genes and both proteins are required for the establishment of actin polarization in A. gossypii germ cells. Agrho1 mutants show a cell lysis phenotype. Null mutant strains of Agrho3 show periodic swelling of hyphal tips that is overcome by repolarization and polar hyphal growth in a manner resembling the germination pattern of spores. Thus different Rho-protein modules are required for distinct steps during polarized hyphal growth of A. gossypii.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
División Celular
/
Proteínas de Unión al GTP rho
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Factores de Intercambio de Guanina Nucleótido
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Proteínas de Saccharomyces cerevisiae
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Saccharomycetales
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Genetics
Año:
2001
Tipo del documento:
Article
País de afiliación:
Suiza
Pais de publicación:
Estados Unidos