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PCR screening for common weak D types shows different distributions in three Central European populations.
Müller, T H; Wagner, F F; Trockenbacher, A; Eicher, N I; Flegel, W A; Schönitzer, D; Schunter, F; Gassner, C.
Afiliación
  • Müller TH; Oldenburg Institute, German Red Cross Blood Donor Service NSOB, Oldenburg, Germany. tmueller@rdg.behringer-ingelheim.com
Transfusion ; 41(1): 45-52, 2001 Jan.
Article en En | MEDLINE | ID: mdl-11161244
BACKGROUND: DNA sequencing showed RHD mutations for all weak D phenotypes investigated in a study from Southwestern Germany. Molecular classification of weak D offers a more reliable basis than serotyping and is relevant for optimal D transfusion strategies. STUDY DESIGN AND METHODS: Sequence-specific primers were designed to detect weak D types 1 to 5 and the partial D phenotype HMi in a modular set for conventional PCR analysis. Alternatively, all reactions were multiplexed into a single tube, and the products were identified after automated capillary electrophoresis by their size and fluorescence. Weak D phenotype samples from 436 donors in the Tyrol (Austria) and Northern Germany were investigated by PCR. RESULTS: More than 90 percent of the weak D types identified by PCR represented type 1, 2, or 3. The distribution among the common types varied between the Tyrol and Northern Germany (p<0.0001). Three new RHD alleles were identified. CONCLUSION: A PCR method of detecting the common weak D types was validated. This PCR system introduces a simple and rapid tool for routine DNA typing of weak D samples. The results confirmed that all weak D phenotype samples identified by current serologic criteria carry altered D proteins.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sistema del Grupo Sanguíneo Rh-Hr / Reacción en Cadena de la Polimerasa / Isoantígenos Tipo de estudio: Diagnostic_studies / Prognostic_studies / Screening_studies Límite: Humans País/Región como asunto: Europa Idioma: En Revista: Transfusion Año: 2001 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sistema del Grupo Sanguíneo Rh-Hr / Reacción en Cadena de la Polimerasa / Isoantígenos Tipo de estudio: Diagnostic_studies / Prognostic_studies / Screening_studies Límite: Humans País/Región como asunto: Europa Idioma: En Revista: Transfusion Año: 2001 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos