Antibody to the nonstructural protein NS1 of Japanese encephalitis virus: potential application of mAb-based indirect ELISA to differentiate infection from vaccination.
Vaccine
; 19(13-14): 1753-63, 2001 Feb 08.
Article
en En
| MEDLINE
| ID: mdl-11166901
ABSTRACT
An indirect enzyme-linked immunosorbent assay (ELISA) was developed to detect and differentiate the antibody responses to Japanese encephalitis (JE) virus nonstructural protein NS1 between infected and vaccinated individuals. The results showed that all convalescent sera from JE patients contained NS1-specific IgG antibodies, while 65 and 40% of these sera showed detectable NS1-specific IgM and IgA antibodies, respectively. Specificity analysis showed that NS1-specific IgM and IgA antibodies from JE patients do not cross-react to dengue virus NS1 glycoprotein, while IgG antibodies from 10% of JE patients showed significant cross-reaction to dengue virus NS1 glycoprotein. To differentiate infection from vaccination, the immune sera from 24 children vaccinated with inactivated JE vaccine were analyzed. The data showed that none of these immune sera had detectable NS1-specific IgG antibodies. The results demonstrated the potential application of JE NS1-specific indirect ELISA to differentiate infection from vaccination.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ensayo de Inmunoadsorción Enzimática
/
Proteínas no Estructurales Virales
/
Encefalitis Japonesa
/
Virus de la Encefalitis Japonesa (Especie)
/
Anticuerpos Monoclonales
/
Anticuerpos Antivirales
Tipo de estudio:
Diagnostic_studies
Límite:
Adult
/
Animals
/
Child
/
Humans
Idioma:
En
Revista:
Vaccine
Año:
2001
Tipo del documento:
Article