Fast and reversible trapping of surface glycine receptors by gephyrin.
Nat Neurosci
; 4(3): 253-60, 2001 Mar.
Article
en En
| MEDLINE
| ID: mdl-11224541
ABSTRACT
Variations in receptor number at a given synapse are known to contribute to synaptic plasticity, but methods used to establish this idea usually do not allow for the determination of the dynamics of these phenomena. We used single-particle tracking to follow in real time, on the cell surface, movements of the glycine receptor (GlyR) with or without the GlyR stabilizing protein gephyrin. GlyR alternated within seconds between diffusive and confined states. In the absence of gephyrin, GlyR were mostly freely diffusing. Gephyrin induced long confinement periods spatially associated with submembranous clusters of gephyrin. However, even when most receptors were stabilized, they still frequently made transitions through the diffusive state. These data show that receptor number in a cluster results from a dynamic equilibrium between the pools of stabilized and freely mobile receptors. Modification of this equilibrium could be involved in regulation of the number of receptors at synapses.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Membranas Sinápticas
/
Proteínas Portadoras
/
Receptores de Glicina
/
Proteínas de la Membrana
Límite:
Animals
Idioma:
En
Revista:
Nat Neurosci
Asunto de la revista:
NEUROLOGIA
Año:
2001
Tipo del documento:
Article
País de afiliación:
Francia