Gene expression profiling of malignant mesothelioma cell lines: cDNA array study.
Int J Cancer
; 91(4): 492-6, 2001 Feb 15.
Article
en En
| MEDLINE
| ID: mdl-11251971
ABSTRACT
To reveal genes relevant for malignant mesothelioma (MM), we carried out cDNA array experiments on 4 MM cell lines and 2 primary mesothelial cell cultures established from pleural fluid of non-cancer patients. Human cancer gene filters including 588 genes were used for the cDNA array experiments. Our study revealed 26 over-expressed genes that play a role in the regulation of cell fate, cell cycle, cell growth and DNA damage repair and 13 under-expressed genes encoding growth factors, receptors and proteins involved in cell adhesion, motility and invasion to be common to 3 or 4 MM cell lines. We confirmed the cDNA array results using RT-PCR for 5 of the over-expressed genes and for 3 of the under-expressed genes. Our study presents gene expression profiles in MM cell lines and shows the involvement of several genes, such as those encoding JAGGED1, ser/thr protein kinase NIK, Ku80 and cyclin D2, novel in MM.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Regulación Neoplásica de la Expresión Génica
/
ADN Complementario
/
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Mesotelioma
Límite:
Humans
Idioma:
En
Revista:
Int J Cancer
Año:
2001
Tipo del documento:
Article
País de afiliación:
Finlandia