Histocompatibility leukocyte antigen-G is not expressed by endometriosis or endometrial tissue.
Fertil Steril
; 75(4): 814-7, 2001 Apr.
Article
en En
| MEDLINE
| ID: mdl-11287041
ABSTRACT
OBJECTIVE:
The immunological mechanisms that support persistence and proliferation of ectopic endometrial implants within the peritoneal cavity of women with endometriosis are unknown. Inhibition of natural killer (NK) and cytotoxic T-cell function has been proposed as a mechanism. We tested the hypothesis that expression of a nonclassical major histocompatibility antigen, HLA-G, might explain the local immunosuppression associated with ectopic endometrium.DESIGN:
Nested case-control study of women with and without laparoscopic evidence of endometriosis.SETTING:
Reproductive endocrinology clinic at a university hospital. PATIENT(S) Peritoneal fluid specimens from 10 women with revised AFS stage I-IV endometriosis and from 10 age-matched normal controls without laparoscopic evidence of endometriosis were tested for the presence of HLA-G protein. Endometriosis and normal endometrial biopsies from four patients were used to prepare stromal cell cultures directly evaluated for HLA-G protein. INTERVENTION(S) None. MAIN OUTCOME MEASURE(S) The expression of HLA-G in peritoneal fluid, tissue, and cell cultures was determined by immunoblotting with a specific monoclonal antibody. RESULT(S) HLA-G protein was not detectable in peritoneal fluid specimens of endometriosis patients or controls. Moreover, ectopic and normal endometrial tissues and stromal cells did not express HLA-G. CONCLUSION(S) Immune cell inhibition in endometriosis must be mediated by factors other than HLA-G.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Antígenos de Histocompatibilidad Clase I
/
Endometriosis
/
Endometrio
/
Antígenos HLA
Tipo de estudio:
Observational_studies
/
Risk_factors_studies
Límite:
Female
/
Humans
Idioma:
En
Revista:
Fertil Steril
Año:
2001
Tipo del documento:
Article
País de afiliación:
Estados Unidos